Method of detecting botulinum neurotoxin and antibodies that neutralize botulinum neurotoxin action
AssigneesWisconsin Alumni Research Foundation, Gary E. Borodic
InventorsEric A. Johnson, Sabine Pellett, William H. Tepp, Gary E. Borodic, David J. Beebe, Amy Paguirigan
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Abstract
A highly sensitive method of analyzing a sample for the presence or activity of botulinum neurotoxin (BoNT) or antibodies specific for botulinum neurotoxin is disclosed. In one embodiment, the method comprises the steps of preparing primary non-human mammalian or avian spinal cord cells, and exposing the cells to a test sample, in parallel with a control sample, and examining the extent of cleavage of the intracellular neuronal target protein in both the test and control sample.
Claims16
A method of analyzing a sample for the presence or activity of botulinum neurotoxin (BoNT) or antibodies specific for botulinum neurotoxin, comprising the following steps:
- a) preparing primary non-human mammalian or avian spinal cord cells in media that contain no serum or growth inhibitors, and
- b) exposing the cells to a test sample, in parallel with a control sample, and examining the extent of cleavage of the intracellular neuronal target protein in both the test and control sample.
Dependencies are inferred from the claim text.