Kits for segregating target nucleic acid from mixed nucleic acid samples
AssigneeFLIR Systems, Inc.
InventorRoger Allyn Forsyth
Life of this patent
Estimating the term…
Abstract
The invention provides methods, compositions and kits for segregating a target nucleic acid from a mixed nucleic acid sample. The methods, compositions and kits comprise a non-processive endonuclease (e.g., a restriction enzyme) or an antibody that binds the target nucleic acid (e.g., has methylation specificity). The mixed nucleic acid sample can comprise prokaryotic and eukaryotic nucleic acid and/or nucleic acid from more than one prokaryotic or eukaryotic organisms.
Claims30
A kit for segregating a target nucleic acid from a mixed sample containing a target nucleic acid and a non-target nucleic acid, comprising:
- (i) a non-processive endonuclease that binds to the target nucleic acid, but does not cleave the target nucleic acid;
- and
- (ii) a buffer having conditions suitable for the non-processive endonuclease to bind the target nucleic acid, but not cleave the target nucleic acid;
- wherein the buffer contains a Ca2+ concentration of at least 50 mM.
A kit for segregating a target nucleic acid from a mixed sample containing a target nucleic acid and a non-target nucleic acid, comprising:
- (i) a non-processive endonuclease that binds to the target nucleic acid, but does not cleave the target nucleic acid;
- and
- (ii) a buffer having conditions suitable for the non-processive endonuclease to bind the target nucleic acid, but not cleave the target nucleic acid;
- wherein the buffer contains a Mg2+ concentration of less than 10 mM.
A kit for segregating a target nucleic acid from a mixed sample containing a target nucleic acid and a non-target nucleic acid, comprising:
- (i) a non-processive endonuclease that binds to the target nucleic acid, but does not cleave the target nucleic acid;
- wherein the non-processive endonuclease comprises a detectable label;
- and
- (ii) a buffer having conditions suitable for the non-processive endonuclease to bind the target nucleic acid, but not cleave the target nucleic acid.
Dependencies are inferred from the claim text.