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AssigneePiere Jouan Biotechnologies S.A.
InventorsJean-Louis Maubois, Jacques Fauquant, Pierre Jouan, Michel Bourtourault
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The invention concerns a method for obtaining a highly enriched TGF-beta protein fraction in activated form, from a liquid solution rich in proteins said to be soluble in the aqueous phase of milk and/or of whey, said method comprising the following steps; a) adjusting soluble proteins purified at a concentration between 5 and 30 g/liter of solution; b) precipitating part of the whey proteins by acidic treatment of the solution thus obtained to a pH ranging between 4 and 5.5 and at a temperature ranging between 55° C. and 68° C.; c) carrying out a microfiltration of the treated solution by diafiltration, so as to obtain respectively a microfiltration retentate and a microfiltrate; d) recuperating the microfiltration retentate containing the protein fraction highly enriched in TGF-beta; e) drying the microfiltration retentate which has been subjected to diafiltration to obtain a powder highly enriched in TGF-beta.
A protein fraction enriched in TGFβ2 in an activated form, said protein fraction comprising a) TGFβ2 in an activated form in a content higher than 5 micrograms per gram of total proteins of said protein fraction, b) whey proteins comprising α-lactalbumin being about 45-80% by weight of said protein fraction, and c) an immunoglobulin being about 10-25% by weight of said protein fraction, wherein said protein fraction is effective for treating psoriasis, wherein said protein fraction is obtained by the steps of:
Dependencies are inferred from the claim text.