Neural transfection reagents
AssigneeInnovative Surface Technologies, Inc.
InventorsEric Guire, Jie Wen
Life of this patent
Estimating the term…
Abstract
The invention is directed to transfection reagents for the delivery of nucleic acids into neural cells, compositions including the reagents, methods of preparation of such reagents, methods of transfecting cells with such reagents, and uses thereof. In preferred embodiments the reagents comprise horseradish peroxidase and/or a polycarboxylic acid such as poly(acrylic acid) or poly(methacrylic acid).
Claims16
A transfection reagent comprising
- (a) a neural transport agent comprising an enzyme, polymer, lectin, fluorescent dye, dextran, or a combination of any two or more of these, and
- (b) a nucleic acid carrier disposed within and conjugated to the neural transport agent,
- wherein the nucleic acid carrier and the neural transport agent comprise complementary chemically reactive groups or high affinity ligands to conjugate the carrier and the transport agent, and
- wherein the transfection reagent has a net negative surface charge under mammalian physiological conditions and an overall effective diameter of 5 to 250 nm.
A composition for transfection of neurons, the composition comprising a neural transport agent comprising an enzyme, polymer, lectin, fluorescent dye, dextran, or a combination of any two or more of these and a nucleic acid carrier, wherein the neural transport agent and the nucleic acid carrier are conjugated to each other via complementary chemically reactive groups or high affinity ligands, and wherein, once conjugated, the composition has a net negative surface charge under mammalian physiological conditions and an overall effective diameter of 5 to 250 nm or less.
Dependencies are inferred from the claim text.