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US 8,935,880 B2Granted
Methods for manipulating yield of plants and identifying yield genes
AssigneeMonsanto Technology LLC
InventorsDaniel J. Ovadya, Bala Karunanandaa, Karen Gabbert, Qin Zeng
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Abstract
Methods for manipulating yield and generation time of plants, especially short day plants such as soybean are provided. The methods comprise manipulating external signals such as long day conditions, short day conditions, growth medium, and nutrient supply.
Claims24
A method for manipulating seed yield of a short day plant, the method comprising:
- (i) planting a seed of a short day plant and growing the plant under long day growing conditions comprising at least about 14 uninterrupted hours of light per day at a light intensity from about 1000 to about 2000 μmoles m−2s−1 until the plant has reached a growth stage of about V1 to V4;
- (ii) after the plant has reached a growth stage of about V1 to V4, growing the plant under short day growing conditions for about 3 to about 21 days;
- (iii) after growing the plant under short day growing conditions for about 3 to about 21 days, growing the plant under said long day growing conditions;
- and
- (iv) allowing seed to form, wherein more of said seed forms relative to a control plant grown for the same amount of time but without all of said steps (i)-(iii).
2 more elements
A method for manipulating seed yield of a soybean plant, the method comprising:
- (i) planting a seed of a soybean plant and growing the plant under long day growing conditions comprising at least about 14 uninterrupted hours of light per day at a light intensity from about 1000 to about 2000 μmoles m−2s−1 until the plant has reached a growth stage of about V1 to V4;
- (ii) after the soybean plant has reached a growth stage of about V1 to V4, growing the plant under short day growing conditions for about 3 to about 21 days;
- (iii) after growing the soybean plant under short day growing conditions for about 3 to about 21 days, growing the soybean plant under said long day growing conditions;
- and
- (iv) allowing seed to form on the plant, wherein more of said seed is formed than a control soybean plant grown for the same amount of time but without all of said steps (i)-(iii).
2 more elements
A method for identifying genes conferring increased seed yield in a short day plant, the method comprising:
- (i) planting a seed of a short day plant and growing the plant under long day growing conditions comprising at least about 14 uninterrupted hours of light per day at a light intensity from about 1000 to about 2000 μmoles m−2s−1 until the plant has reached a growth stage of about V1 to V4;
- (ii) after the plant has reached a growth stage of about V1 to V4, growing it under short day growing conditions for about 3 to about 21 days;
- (iii) after growing the plant under short day growing conditions for about 3 to about 21 days, growing the plant under said long day growing conditions;
- harvesting tissue from the short day plant;
- and
- performing transcriptional profiling on the harvested tissue to identify genes that may confer increased seed yield in the short day plant.
3 more elements
Dependencies are inferred from the claim text.