Method for measuring solubility
AssigneeAnaliza, Inc.
InventorsArnon Chait, Boris Y. Zaslavsky
Life of this patent
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Abstract
A method of determining the solubility of a compound in a selected solvent is provided that does not require determination of, or use of, standards having known concentrations of the compound. In one aspect, the method can include preparation of a mixture where not all of a compound is dissolved in the provided solvent, separating undissolved compound from the solvent, and direct determination of the amount of the compound dissolved in the solvent. Methods adapted for use include those where a multiplicity of compounds or solvents are tested in parallel. Devices adapted for these methods are also provided by the present disclosure.
Claims52
A method for determining the solubility of a solid compound in a solvent, the method comprising the steps of:
- (a) preparing a mixture of a quantity of the solid compound with a volume of the solvent;
- (b) incubating the mixture of the compound and the solvent to form a solution of the compound, whereby a first portion of the compound dissolves in the solvent to form the solution of the compound, and a second portion of the compound remains undissolved as a solid;
- (c) removing the undissolved solid compound from the mixture, thereby providing a quantity of the solution of the compound;
- (d) determining an amount of a constituent in the quantity of the solution of the compound resulting from (c), wherein the determination does not include a comparison of physical properties of one or more solutions containing known concentrations of the compound and physical properties of the solution of the compound;
- and
- (e) calculating solubility of the compound in the solvent by determining an amount of the compound dissolved in the quantity of the solution of the compound based on the determination of the amount of the constituent in (d).
A method for determining the solubility of a solid compound in a solvent, the method comprising the steps of:
- (a) preparing a mixture of a quantity of the solid compound with a volume of the solvent;
- (b) incubating the mixture of the compound and the solvent to form a solution of the compound, whereby a first portion of the compound dissolves in the solvent to form the solution of the compound, and a second portion of the compound remains undissolved;
- (c) removing the undissolved compound from the mixture, thereby providing a quantity of the solution of the compound;
- (d) determining an amount of nitrogen and/or sulfur in the quantity of the solution of the compound resulting from (c) using a universal equimolar chemiluminescent detector;
- (e) calculating solubility of the compound in the solvent by determining an amount of the compound dissolved in the quantity of the solution of the compound, based on the determination of the amount of nitrogen and/or sulfur in (d).
A high-throughput method for determining the solubilities of a plurality of solid compounds, each in a solvent, the method comprising the steps of:
- (a) for each solid compound of a plurality of solid compounds, automatically dispersing the solid compound as a dry powder in a volume of a solvent;
- (b) incubating each of the mixtures of the respective compounds and the solvent to form a plurality of solutions of each compound, whereby a first portion of each of the compounds dissolves in the solvent to form the respective solutions of each of the compounds, and a second portion of each of the compounds remains undissolved;
- (c) removing the undissolved compounds from each of the mixtures, thereby providing a plurality of solutions for each of the compounds;
- (d) determining amounts of a constituent in each of the plurality of solutions resulting from (c), wherein the determinations do not include a comparison of physical properties of one or more solutions containing known concentrations of each respective compound and physical properties of the solution of each respective compound;
- and
- (e) calculating solubilities of each respective compound in the solvent by determining amounts of each compound dissolved in the respective solutions, based on the determination of the amounts of the constituent in (d).
Dependencies are inferred from the claim text.