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AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsTheodore S Jardetzky, Beomkyu Kim
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Embodiments of the invention are related to a polypeptide comprising the amino acid sequence of a human IgE-Fc Cε3-Cε4, wherein said Cε3-Cε4 starts at amino acid 328 and ends at amino acid 547 of said IgE-Fc, and wherein C 328 is A and K 367 is C. Other embodiments concern a second polypeptide comprising the amino acid sequence of a human FcεRIα extracellular region, wherein said extracellular region starts at amino acid 1 and ends at amino acid 176 of said FcεRIα. Still other embodiments are related to a method of identifying a compound that inhibits the binding of an IgE-Fc to a FcεRIα, said method comprising: contacting the polypeptide, wherein said IgE-Fc Cε3-Cε4 sequence is labeled with a fluorophore, and the second polypeptide, with a test compound; and determining whether binding of said polypeptide to said second polypeptide is decreased in the presence of said test compound.
A polypeptide comprising an amino acid sequence that is at least identical to the amino acid sequence of a human IgE-Fc Cε3-Cε4, wherein said Cε3-Cε4 starts at amino acid 328 and ends at amino acid 547 of said IgE-Fc, using the numbering according to the reference sequence of SEQ ID NO:14, and wherein C 328 is an amino acid other than C and K 367 is C, and wherein the polypeptide is capable of binding to an FcεRIα.
A polypeptide comprising the amino acid sequence of a human IgE-Fc Cε3-Cε4, wherein said Cε3-Cε4 starts at amino acid 328 and ends at amino acid 547 of said IgE-Fc, using the numbering according to the reference sequence of SEQ ID NO:14, but with up to 30% insertions, deletions, or conservative substitutions, and wherein C 328 is an amino acid other than C and K 367 is C, and wherein the polypeptide is capable of binding to an FcεRIα.
A polypeptide comprising the amino acid sequence of a human IgE-Fc Cε3-Cε4, wherein said Cε3-Cε4 starts at amino acid 328 and ends at amino acid 547 of said IgE-Fc, using the numbering according to the reference sequence of SEQ ID NO:14, and wherein C 328 is A and K 367 is C.
Dependencies are inferred from the claim text.