Method for diagnosing immunoglobulin A nephropathy and TGBM nephropathy

A method of detecting IgA nephropathy in a patient suspected of having IgA nephropathy using urine exosome from said patient comprising:

• obtaining a urine sample from said patient;
• separating exosome from said urine sample;
• performing an immunoassay on said urine exosome comprising reacting said urine exosome with isolated monoclonal or polyclonal antibodies specifically binding to at least three different proteins selected from the group consisting of Ceruloplasmin precursor having Accession No. IPI00017601.1, Alpha-1-antitrypsin precursor having Accession No. IPI00553177.1, Serotransferrin precursor having Accession No. IPI00022463.1, Transferrin variant Fragment having Accession No. IPI00798430.1, and Alpha-2-macroglobulin precursor having Accession No. IPI00478003.1 to obtain at least three different specifically bound antibody-protein complexes;
• measuring levels of said at least three different specifically bound antibody-protein complexes to determine expression levels of said at least three different proteins in said urine exosome;
• detecting whether said urine exosome contains increased levels of said at least three different proteins by comparing said expression levels of said at least three different proteins in said urine exosome to levels of said at least three different proteins in urine exosome samples from healthy patients;
• and
• diagnosing that said patient has IgA nephropathy if said patient's urine exosome contains significantly higher levels of said at least three different proteins compared to said levels of said at least three different proteins in urine exosome samples from healthy patients.