Virus causing respiratory tract illness in susceptible mammals
AssigneeViroNovative BV
InventorsJan Cornelius De Jong, Ronaldus Adrianus Maria Fouchier, Bernadetta Gerarda Van Den Hoogen, Albertus Dominicus Marcellinus Erasmus Osterhaus, Jan Groen
Life of this patent
Estimating the term…
Abstract
The invention relates to the field of virology. The invention provides an isolated essentially mammalian negative-sense single stranded RNA virus (MPV) within the sub-family Pneumovirinae of the family Paramyxoviridae and identifiable as phylogenetically corresponding to the genus Metapneumovirus and components thereof.
Claims28
A method for identifying a viral isolate from a mammalian subject as comprising a human metapneumovirus, the method comprising:
- reacting the viral isolate or a component thereof with a DNA molecule of at least 10 nucleotides in length that is at least 90% homologous to a stretch of the DNA sequence of SEQ ID NO:
- 36, or the complement thereof;
- wherein reaction of the DNA molecule with the viral isolate or component thereof indicates the presence of human metapneumovirus in the viral isolate from the mammalian subject.
A method for diagnosing a human metapneumovirus infection in a mammal, the method comprising:
- determining in a sample of the mammal the presence of a component of human metapneumovirus by reacting the sample with a DNA molecule of at least 10 nucleotides in length that is at least 90% homologous to a stretch of the DNA sequence of SEQ ID NO:
- 36, or the complement thereof;
- wherein reaction of the DNA molecule with the sample of the mammal indicates human metapneumovirus infection of the mammal.
A method for diagnosing a human metapneumovirus infection of a mammal, the method comprising:
- determining in a sample from the mammal the presence of human metapneumovirus or component thereof by reacting the sample with a DNA molecule specifically reactive with a stretch of at least 10 nucleotides of the DNA sequence of SEQ ID NO:
- 36, or the complement thereof;
- wherein reaction of the DNA molecule with the sample of the mammal indicates human metapneumovirus infection of the mammal.
A method of detecting if a polynucleotide from human metapneumovirus is present in a mammalian subject, the method comprising:
- performing an nucleic acid amplification reaction on a sample derived from the mammalian subject utilizing a set of primers for the amplification of at least a portion of the N gene of human metapneumovirus to create an amplified product, wherein the N gene, when transcribed and translated, produces a polypeptide at least 90% homologous to SEQ ID NO:
- 1;
- contacting the amplified product with a DNA molecule that hybridizes with the amplified product;
- hybridizing the DNA molecule to the amplified product;
- and
- detecting the hybridized DNA molecule;
- wherein the detection of the hybridized DNA molecule indicates the presence of human metapneumovirus in the mammalian subject.
A method of detecting if a polynucleotide from human metapneumovirus is present in a mammalian subject, the method comprising:
- performing an nucleic acid amplification reaction on a sample derived from the mammalian subject utilizing a set of primers for the amplification of at least a portion of the N gene of human metapneumovirus to create an amplified product, wherein the N gene, when transcribed and translated, produces a polypeptide at least 90% homologous to SEQ ID NO:
- 1;
- contacting the amplified product with a DNA molecule of at least 25 nucleotides in length that specifically hybridizes with the amplified product;
- hybridizing the DNA molecule to the amplified product;
- and
- detecting the hybridized DNA molecule;
- wherein the detection of the hybridized DNA molecule indicates the presence of human metapneumovirus in the mammalian subject.
Dependencies are inferred from the claim text.