Methods of using chimeric receptors to identify autoimmune disease
AssigneeDiagnostic Hybrids, Inc.
InventorJames L. Brown
Life of this patent
Estimating the term…
Abstract
The present invention provides methods and compositions useful in the diagnosis and management of autoimmune diseases. In particular, the present invention provides improved methods and compositions for the diagnosis and management of Graves' disease. The methods of the present invention not only avoids the need for radioactivity and are much simpler, economical, and rapid than methods traditionally used for the diagnosis of Graves' disease, but also improve upon the sensitivity and detection abilities of previous luciferase-based autoantibody detection assays.
Claims16
A recombinant chimeric thyroid stimulating hormone (TSH) receptor polypeptide encoded by nucleotides 45-2234 of SEQ ID NO:3.
A recombinant polypeptide consisting of a chimeric thyroid stimulating hormone (TSH) receptor, wherein said chimeric TSH receptor consists of:
- an N-terminal portion of a human thyroid stimulating hormone receptor (hTSHR), wherein the N-terminal portion of the hTSHR consists of the amino acid sequence encoded by nucleotides 45-827 of SEQ NO:3;
- a portion of a rat luteinizing hormone chorionic gonadotropin receptor (rLH/CGR), wherein the portion of the rLH/CGR consists of the amino acid sequence encoded by nucleotides 828 to 1046 of SEQ ID NO:3;
- and
- a C-terminal portion of said hTSHR, wherein the C-terminal portion of the hTSHR consists of the amino acid sequence encoded by nucleotides 1047-2234 of SEQ ID NO:3.
Dependencies are inferred from the claim text.