Acyl-ACP wax ester synthases
AssigneeExxonMobil Research and Engineering Company
InventorsErik Holtzapple, John H. Verruto
Life of this patent
Estimating the term…
Abstract
The invention relates to acyl-CoA-independent methods of producing a wax ester in recombinant host cells engineered to express an acyl-ACP wax ester synthase, and an alcohol-forming acyl-ACP reductase. The methods of the invention may take place in photosynthetic microorganisms, and particularly in cyanobacteria. Isolated nucleotide molecules and vectors expressing an acyl-ACP wax ester synthase and/or an alcohol-forming acyl-ACP reductase, recombinant host cells expressing an acyl-ACP wax ester synthase and optionally an alcohol-forming acyl-ACP reductase, and systems for producing a wax ester via an acyl-CoA-independent pathway, are also provided.
Claims26
A recombinant host cell genetically engineered for the production of fatty acid esters from acyl-ACP, wherein the recombinant host cell comprises a non-native nucleic acid sequence that encodes (1) an acyl-ACP wax ester synthase having a sequence identity of at least 85% to a polypeptide selected from the group consisting of SEQ ID NO:
- 19, SEQ ID NO:
- 21, SEQ ID NO:
- 23, SEQ ID NO:
- 25, SEQ ID NO:
- 27, SEQ ID NO:
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- 33, SEQ ID NO:
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- 41, and SEQ ID NO:
- 43 and (2) a non-native nucleic acid sequence that encodes an alcohol-forming acyl-ACP reductase having a sequence identity of at least 85% to a polypeptide selected from the group consisting of SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, and SEQ ID NO:
- 10, wherein each non-native nucleic acid sequence is operably linked to a promoter, and wherein at least one of the non-native nucleic acids is heterologous relative to the host cell or to the promoter to which the non-native nucleic acid sequence is operably linked, and further wherein the recombinant host cell does not express one or more of:
- a) an exogenous acyl-ACP thioesterase;
- b) an exogenous acyl-CoA thioesterase;
- and
- c) an exogenous acyl-CoA synthetase.
Dependencies are inferred from the claim text.