Method for the urinary detection of bladder cancer
AssigneeArray Genomics
InventorsOlivier Cussenot, Ian Jones, Neil Metters, François Lozach
Life of this patent
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Abstract
The invention relates to the diagnosis of bladder cancer and more specifically to the detection in urine samples of bladder carcinomas of the transitional type. The detection method according to the present invention enables, through the utilization of a DNA chip designed for this purpose, to determine the grade of the detected tumors.
Claims33
A method for in-vitro detection of bladder cancer, and deriving the stage of the cancer in a patient, comprising:
- (i) extracting DNA contained in a urine sample taken from said patient;
- (ii) fragmenting the DNA extracted In step (I);
- (iii) marking the obtained DNA fragments uniformly with a marking agent so as to form a pool of marked DNA;
- (iv) forming at least one aliquot from the pool of marked DNA and bringing each aliquot into contact with a set of reference DNAs, said contact being carried out under conditions enabling specific hybridization of the marked DNA fragments with said reference DNAs;
- wherein the set of reference DNAs consists essentially of sequences included in each of the following loci of the human chromosome:
- 1p, 3q, 8q22qter, 20, 5p12-p13, 9p, 9q, 18q12, 1q22-q24, 5p, 6q22, 7, 11q13, 12q15, 13q, 15, 16, 17q, 6q25-q27, 7q, 8p, 10q, 11p, 14q22-qter, 17p, 19 and 22, and wherein the reference DNAs are at least 30 bases in length;
- (v) eliminating the marked DNA fragments which are not specifically hybridized to the reference DNAs;
- (vi) determining the intensity of the signal produced by the marked fragments hybridized to each of the selected reference DNAs;
- (vii) determining, for each reference DNA, the deviations between the signals obtained In comparison with those obtained with a control DNA from a healthy patient;
- and
- (viii) deriving the patient's cancer stage from said observed deviations.
A DNA microarray for the detection of bladder cancer comprising, fixed on its surface, deposits of 10 to 1000 reference DNAs;
- said reference DNAs having sequences included in each of the following loci of the human chromosome:
- 1p, 3q, 8q22qter, 20, 5p12-p13, 9p, 9q, 18q12, 1q22-q24, 5p, 6q22, 7, 11q13, 12q15, 13q, 15, 16, 17q, 6q25-q27, 7q, 8p, 10q, 11p, 14q22-qter, 17q, 19 and 22, and wherein the reference DNAs are at least 30 bases in length.
A DNA microarray comprising, fixed on its surface, reference DNA deposits consisting essentially of DNA sequences of the following loci of the human chromosome:
- 1p, 3q, 8q22qter, 20, 5p12-p13, 9p, 9q, 18q12, 1q22-q24, 5p, 6q22, 7, 11q13, 12q15, 13q, 15, 16, 17q, 6q25-q27, 7q, 8p, 10q, 11p, 14q22-qter, 17p, 19 and 22;
- and wherein the DNA sequences are at least 30 bases in length.
Dependencies are inferred from the claim text.