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US 8,914,240 B2Granted
Method for determining coronary artery disease risk
AssigneeCardiodx, Inc.
InventorsSteven Rosenberg, Michael R. Elashoff, Philip Beineke, James A. Wingrove, Whittemore G. Tingley, Susan E. Daniels
Life of this patent
Estimating the term…
Abstract
Markers and methods useful for assessing coronary artery disease in a subject are provided, along with kits for measuring their expression. Also provided are predictive models, based on the markers, as well as computer systems, and software embodiments of the models for scoring and optionally classifying samples.
Claims7
A method for determining coronary artery disease risk in a subject comprising:
- (i) performing a reverse transcriptase polymerase chain reaction (RT-PCR) assay on a sample from the subject by using a plurality of distinct primer and probe sets that specifically hybridize to mRNA corresponding to each of the following genes consisting of:
- S100A12, CLEC4E, S100A8, CASP5, IL18RAP, TNFAIP6, AQP9, NCF4, CD3D, TMC8, CD79B, SPIB, HNRPF, TFCP2, RPL28, AF161365, AF289562, SLAMF7, KLRC4, IL8RB, TNFRSF 10C, KCNE3, and TLR4;
- (ii) generating, based on said assay, data representing mRNA expression levels corresponding to each of said genes;
- (iii) obtaining data representing age of the subject and gender of the subject;
- and (iv) generating, by a computer processor, a score indicative of coronary artery disease (CAD) risk by mathematically combining the data representing the mRNA expression levels, and the data representing the age and gender of the subject, wherein a higher score relative to a control subject having <50% stenosis in all major vessels indicates an increased likelihood that the subject has CAD.
2 more elements
Dependencies are inferred from the claim text.