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US 8,912,511 B2Granted
Device and method for multi-photon fluorescence microscopy for obtaining information from biological tissue
AssigneeW.O.M. World of Medicine GmbH
InventorKarl-Heinz Guenter Schoenborn
Life of this patent
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Abstract
A device for multi-photon fluorescence microscopy for obtaining information from biological tissue is provided. The device comprising a laser unit for generating an excitation radiation, an optical unit which is formed to focus the excitation radiation for generating an optical signal at different locations in or on an object to be examined, and a detector module for detecting the optical signal from the region of the object. The optical unit for generating the optical signal at different locations in or on the object is movable in at least one direction relative to the object.
Claims14
A device for multi-photon fluorescence microscopy for obtaining information from biological tissue, comprising:
- a laser unit for generating an excitation radiation,
- an optical unit which is formed to focus the excitation radiation for generating an optical signal at different locations in or on an object to be examined,
- a control and processing unit,
- a patient module connected to the control and the processing unit, which for examining the object can be placed relative to the object, wherein the optical is part of the patient module, and
- a detector module for detecting the optical signal from the region of the object,
- wherein the optical unit for generating the optical signal at different locations in or on the object is movable within a housing of the patient module in at least one direction relative to the object,
- wherein the laser unit is part of the control and processing unit and an optical fiber connects the laser unit with the patient module for transmitting the excitation radiation towards the optical unit,
- wherein the laser unit generates an excitation radiation with a first wavelength to transmit the excitation radiation through the optical fiber, and a frequency doubler is arranged in the patient module for halving the wavelength of the excitation radiation prior to the optical unit,
- wherein from the received optical signal is a sectional image of the object is generated pixel by pixel at different locations of the object,
- wherein for generating the sectional image pixel by pixel the object is exposed to the excitation radiation in a triggered manner, and
- wherein the pixel size of the sectional image pixel by pixel is determined in horizontal direction by the focus of the excitation radiation and by adjusting the triggering, and in vertical direction by the waist length of the focused excitation radiation,
- wherein the pixel size is adjustable by a beam expansion of the excitation radiation and by adjusting the triggering.
9 more elements
A method for multi-photon fluorescence microscopy for obtaining information from biological tissue, in which
- a laser unit generates an excitation radiation,
- an optical unit focuses the excitation radiation for generating an optical signal at different locations in or on an object to be examined,
- a control and processing unit,
- a patient module connected to the control and the processing unit, which for examining the object can be placed relative to the object, wherein the optical is part of the patient module, and
- a detector module detects the optical signal from the region of the object,
- wherein the optical unit for generating the optical signal in or on the object is moved within a housing of the patient module in at least one direction relative to the object,
- wherein the laser unit is part of the control and processing unit and an optical fiber connects the laser unit with the patient module for transmitting the excitation radiation towards the optical unit, and
- wherein the laser unit generates an excitation radiation with a first wavelength to transmit the excitation radiation through the optical fiber, and a frequency doubler is arranged in the patient module for halving the wavelength of the excitation radiation prior to the optical unit,
- wherein from the received optical signal is a sectional image of the object is generated pixel by pixel at different locations of the object,
- wherein for generating the sectional image pixel by pixel the object is exposed to the excitation radiation in a triggered manner, and
- wherein the pixel size of the sectional image pixel by pixel is determined in horizontal direction by the focus of the excitation radiation and by adjusting the triggering, and in vertical direction by the waist length of the focused excitation radiation,
- wherein the pixel size is adjustable by a beam expansion of the excitation radiation and by adjusting the triggering.
9 more elements
Dependencies are inferred from the claim text.