Transcriptional regulation of plant disease tolerance
AssigneeMendel Biotechnology, Inc.
InventorsKaren S. Century, Emily L. Queen, T. Lynne Reuber, Oliver Ratcliffe, Roger D. Canales, Neal I. Gutterson
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Abstract
The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties, including disease stress and abiotic stress tolerance, as compared to wild-type or control plants. The invention also pertains to expression systems that may be used to regulate these transcription factor polynucleotides, providing constitutive, transient, inducible and tissue-specific regulation.
Claims16
A method for increasing a plant's tolerance or resistance to a fungal pathogen, the method comprising:
- (a) providing an expression vector comprising a regulatory element and a polynucleotide sequence;
- wherein the polynucleotide encodes a polypeptide comprises SEQ ID NO:
- 6, and
- the polynucleotide sequence is operably linked to the regulatory element, and the regulatory element controls expression of the polynucleotide sequence;
- and
- (b) transforming a target plant with the expression vector to produce a transformed plant, wherein the polypeptide is overexpressed in the transformed plant, and said overexpression results in the transformed plant having greater tolerance or resistance to a fungal disease as compared to a control plant;
- (c) growing the plant in an environment that contains at least a fungal pathogen;
- and
- (d) selecting a transgenic plant having greater tolerance or resistance to the fungal pathogen as compared to the control plant as a result of expression of the polypeptide.
A method for producing a plant with greater tolerance or resistance to a fungal pathogen than a control plant, the method comprising:
- (a) providing an expression vector comprising a regulatory element and a polynucleotide sequence;
- wherein
- the polynucleotide encodes a polypeptide that comprises SEQ ID NO:
- 6;
- the polynucleotide sequence is operably linked to the regulatory element, and the regulatory element controls expression of the polynucleotide sequence;
- and
- (b) transforming a target plant with the expression vector to produce a transformed plant, wherein the polypeptide is overexpressed in the transformed plant, and said overexpression results in the transformed plant having greater fungal disease tolerance or resistance than the control plant;
- and
- (c) growing the plant in an environment that contains a fungal pathogen.
A transgenic plant comprising an expression vector comprising:
- (i) a polynucleotide sequence encoding SEQ ID NO:
- 6;
- and
- (ii) a regulatory element that controls expression of the polynucleotide sequence.
Dependencies are inferred from the claim text.