Method for the determination of the DNA methylation level of a CPG position in identical cells within a tissue sample
AssigneeEpigenomics AG
InventorsJoern Lewin, Kurt Berlin
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Abstract
Aspects of the present invention relate to the determination of the DNA methylation level at one or more CpG position within cells of a defined type in a tissue sample. This methylation level is deduced from the total DNA methylation level of all cells of the sample and from the content of said cells of interest. In aspects of the invention, the cell content is determined by means of histopatholoy, staining methods, antibodies, expression analysis or DNA methylation analysis.
Claims17
Method for the determination of the DNA methylation level of one or more specific CpG positions of a subpopulation of cells within a tissue sample comprising a plurality of subpopulations of cells, comprising
- a) determining, in a quantitative or semi-quantitative manner, the proportion of cells within said tissue sample represented by said subpopulation of cells,
- b) measuring the total DNA methylation level of said one or more specific CpG positions within said tissue sample,
- c) determining the DNA methylation level within the cells of said subpopulation of cells based on the total DNA methylation level of said one or more specific CpG positions within the tissue sample and the proportion of cells within said tissue sample represented by said subpopulation.
Dependencies are inferred from the claim text.