Carbohydrate chip for detection of pathogen Vibrio cholerae and method of preparing the same
AssigneePostech Academy-Industry Foundation
InventorsHyung Joon Cha, Chang Sup Kim, Jeong Hyun Seo, Hwahui Shin
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Abstract
The present invention related to a saccharide-based cholera toxin detection sensor for detection of Vibrio cholerae and its use. More specifically, the present invention relates to a carbohydrate chip for detection of Vibrio cholerae, a method for detecting Vibrio cholerae using the same, and a method for preparing the same, where the carbohydrate chip comprises GM1 pentasaccharide, GM2 tetrasaccharide, asialo GM1 tetrasaccharide, GM3 trisaccharide, galactose-β 1,3-N-acetylgalactosamine, lactose, and sialic acid that are immobilized on the surface of a solid substrate.
Claims6
A method for detecting Vibrio cholerae by detecting cholera toxin consisting of one A subunit and five B subunits, the method comprising:
- applying a sample containing cholera toxin onto a carbohydrate chip,
- wherein the carbohydrate chip comprises (i) a solid substrate and (ii) GM1 pentasaccharide, GM2 tetrasaccharide, asialo GM1 tetrasaccharide, GM3 trisaccharide, galactose-β 1,3-N-acetylgalactosamine, lactose, and sialic acid immobilized on a surface of the solid substrate,
- wherein the carbohydrate chip has a visual limit of detection (LOD) of 5 ng/mL for cholera toxin produced by Vibrio cholera;
- and
- detecting the cholera toxin by fluorescence image analysis.
Dependencies are inferred from the claim text.