Method of generating gene mosaics
AssigneeEviagenics S.A.
InventorsRudy Pandjaitan, Alejandro Luque
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Abstract
The invention relates to a method for generating a gene mosaic by somatic in vivo recombination, comprising: e) in a single step procedure (vii) transforming a cell with at least one gene A having a sequence homology of less than 99.5% to another gene to be recombined that is an integral part of the cell genome or presented in the framework of a genetic construct, (viii) recombining said genes, (ix) generating a gene mosaic of the genes at an integration site of a target genome, wherein said at least one gene A has a single flanking target sequence either at the 5′ end or 3′ end anchoring to the 5′ or 3′ end of said integration site, and f) selecting clones comprising the gene mosaic, as well as a method of producing a diversity of gene mosaics and gene assembly.
Claims20
A method for generating a gene mosaic by somatic in vivo recombination, comprising:
- a) in a single step procedure:
- (i) transforming a repair deficient host cell with at least a first gene having a sequence homology of less than 99.5% to a second gene to be recombined with the first gene, the second gene being an integral part of the host cell genome or present in a framework of a genetic construct in the host cell,
- (ii) recombining said genes, and
- (iii) generating a gene mosaic from the first and second genes, the gene mosaic being integrated into a chromosome of the host cell at an integration site of a target genome, wherein the first gene has a single flanking target sequence either at the 5′ end or 3′ end anchoring to the 5′ or 3′ end of said integration site, and
- b) selecting clones comprising the gene mosaic.
Dependencies are inferred from the claim text.