Microfluidic device for generating neural cells to simulate post-stroke conditions
AssigneesThe Charles Stark Draper Laboratory, Inc., University of South Florida
InventorsAnilkumar Harapanahalli Achyuta, Javier Cuevas, Shivshankar Sundaram, Chris Katnik
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Abstract
This application provides devices for modeling ischemic stroke conditions. The devices can be used to culture neurons and to subject a first population of the neurons to low-oxygen conditions and a second population of neurons to normoxic conditions. The neurons are cultured on a porous barrier, and on the other side of the barrier run one or more fluid-filled channels. By flowing fluid with different oxygen levels through the channels, one can deliver desired oxygen concentrations to the cells nearest those channels.
Claims18
A device comprising:
- a fluid-containing chamber housing cells, wherein the cells comprise a first population and a second population;
- a porous barrier to which the cells are adhered;
- a first channel having an inlet and an outlet, separated from the chamber and the cells by the porous barrier, wherein the first population is located proximate to the first channel, relative to the second population, which is located further away than the first population from the first channel;
- a first array of electrodes positioned between the first channel and the porous barrier and aligned along a length of the first channel;
- and
- a second array of electrodes disposed on a fluid facing side of a roof of the fluid-containing chamber, wherein the roof is opposite to and parallel with the porous barrier.
Dependencies are inferred from the claim text.