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US 8,912,004 B2Granted
Optical sensor-based cupric reducing antioxidant capacity (CUPRAC) assay
AssigneeOxford Biomedical Research, Inc.
InventorsMustafa Resat Apak, Kubilay Guclu, Mustafa Ozyurek, Mustafa Bener, Enrique Martinez, Denis Callewaert
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Abstract
An example embodiment is an apparatus for measuring antioxidant presence comprising a solid media, and a reagent carried by the media, the reagent reactive with at least one antioxidant to cause changes in light that is incident upon the reagent while carried by the solid media.
Claims9
An apparatus for measuring antioxidant capacity in a biological sample comprising:
- a solid media, wherein the solid media is composed of paper or woven fabrics;
- and,
- a reagent carried by the media, wherein the reagent is comprised of copper and neocuproine (2,9-dimethyl-1,10-phenanthroline, abbreviated as Nc) chelator embedded in or adsorbed to the solid media and wherein the reagent is reactive with a number of antioxidants in a biological sample to cause changes in light that are incident upon the reagent while carried by the solid media, wherein the reagent has a standard reduction potential of about 0.6V.
A solid phase antioxidant assay apparatus comprising:
- a solid phase matrix, wherein the solid phase matrix is solid media composed of paper or woven fabrics;
- an impregnated in the solid media copper(II)-neocuproine reagent that oxidizes one or more antioxidants as they contact the reagent, the copper(II)-neocuproine reagent undergoing a change through the copper(II)-neocuproine reduction that causes changes in light reflected from the immobilized reagent, with the changes being proportional with antioxidant concentration over a certain antioxidant concentration range, wherein the reagent has a standard reduction potential of about 0.6V;
- a light source for illuminating the impregnated solid media;
- a light sensor for determining a measurement of light reflected from the impregnated solid media;
- and,
- a reference for comparing the measurement to, the comparison useful to indicate concentration of the antioxidant.
3 more elements
Dependencies are inferred from the claim text.