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US 8,911,997 B2Granted
Mammalian cell culture medium
AssigneeQueensland University of Technology
InventorsZee Upton, Damien Harkin, David Leavesley
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Abstract
A cell culture medium and system are provided which eliminate or at least reduce the requirement for exogenous components such as serum and feeder cells. The cell culture medium comprises an IGF and vitronectin or fibronectin and, optionally an IGFBP, and is particularly suitable for propagating keratinocytes for subsequent use in skin growth and regeneration. This invention also relates to compositions and methods for skin growth and regeneration in situ, which utilize aerosol delivery of cultured keratinocytes.
Claims17
A mammalian cell culture medium comprising:
- (i) a synthetic, chimeric oligo-protein complex comprising a fused contiguous sequence of amino acids comprising:
- (a) insulin-like growth factor I (IGF-I), and
- (b) an αv integrin-receptor binding domain of vitronectin (VN), wherein the binding domain does not comprise a heparin binding domain (HBD);
- and
- wherein the synthetic, chimeric oligo-protein complex does not comprise an insulin-like growth factor binding protein (IGFBP);
- and
- (ii) an absence of serum or an amount of serum which would not support cell growth when said IGF-I is not present.
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Dependencies are inferred from the claim text.