Method for the preparation of hydroxy acids
AssigneeMetabolic Explorer
InventorsPhilippe Soucaille, Cedric Boisart
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Abstract
The present invention concerns a modified microorganism for the biological preparation of an hydroxy acid of formula (I) wherein the microorganism comprises a two-step metabolic pathway for the production of the said hydroxy acid from a hydroxy keto-acid of formula (II) through an intermediate hydroxy-aldehyde of formula (III), wherein EA1 is an enzyme having a 2-keto-acid decarboxylase activity, and EA2 is an enzyme having hydroxy aldehyde dehydrogenase activity. The invention also concerns a method for the fermentative production of a hydroxy acid.
Claims14
A microorganism genetically modified for the bioproduction of a hydroxy acid of formula (I)
- wherein the microorganism comprises a two-step metabolic pathway for said hydroxy acid from a hydroxy-2-keto-aliphatic acid of formula (II) through an intermediate hydroxy-aldehyde of formula (III):
- with n =0, 1, or 2,
- wherein
- EA1 is an enzyme having a 2-keto-acid decarboxylase activity, and
- EA2 is an enzyme having hydroxy aldehyde dehydrogenase activity, wherein said enzyme EA1 is encoded by a heterologous gene, and wherein producing said hydroxy-2-keto-aliphatic acid of formula (II) is increased in said microorganism by increasing the level of expression of at least one of the following enzymes:
- serine transaminase, serine oxidase, 3-phosphohydroxypyruvate phosphatase, homoserine transaminase, homoserine oxidase, 4-oxoglutaryl-CoA synthetase, and alcohol dehydrogenase.
Dependencies are inferred from the claim text.