Back to results
US 8,911,977 B2Granted
Enzymes useful for peracid production
AssigneeE. I. Du Pont de Nemours and Company
InventorsMark Scott Payne, Robert DiCosimo
Life of this patent
Estimating the term…
Abstract
Acetyl xylan esterases and variants thereof having perhydrolytic activity are provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. Multi-component peracid generation systems comprising an enzyme catalyst having perhydrolytic activity are also provided, as are methods of using the present enzyme catalyst to produce peroxycarboxylic acids. The polypeptide having perhydrolytic activity may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, paper pulp processing, and personal care applications.
Claims18
A process for producing a peroxycarboxylic acid comprising:
- (a) providing a set of reaction components comprising:
- (1) at least one substrate selected from the group consisting of:
- (i) one or more esters having the structure
- [X]mR5
- wherein
- X=an ester group of the formula R6—C(O)O;
- R6=a C1 to C7 linear, branched or cyclic hydrocarbyl moiety, optionally substituted with hydroxyl groups or C1 to C4 alkoxy groups, wherein R6 optionally comprises one or more ether linkages for R6=C2 to C7;
- R5=a C1 to C6 linear, branched, or cyclic hydrocarbyl moiety or a five-membered cyclic heteroaromatic moiety or six-membered cyclic aromatic or heteroaromatic moiety optionally substituted with hydroxyl groups;
- wherein each carbon atom in R5 individually comprises no more than one hydroxyl group or no more than one ester group or carboxylic acid group;
- wherein R5 optionally comprises one or more ether linkages;
- m=an integer ranging from 1 to the number of carbon atoms in R5;
- and wherein said esters have solubility in water of at least 5 ppm at 25° C.;
- (ii) one or more glycerides having the structure
- wherein R1=a C1 to C21 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R3 and R4 are individually H or R1C(O);
- (iii) one or more esters of the formula:
- wherein R1=a C1 to C7 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R2=a C1 to C10 straight chain or branched chain alkyl, alkenyl, alkynyl, aryl, alkylaryl, alkylheteroaryl, heteroaryl, (CH2CH2O)n, or (CH2CH(CH3)—O)nH and n is 1 to 10;
- (iv) one or more acylated monosaccharides, acylated disaccharides, or acylated polysaccharides;
- and
- (v) any combination of (i) through (iv);
- (2) a source of peroxygen;
- and
- (3) an enzyme catalyst comprising a polypeptide having perhydrolytic activity and an amino acid sequence having at least 90% identity to the amino acid sequence set forth in SEQ ID NO:
- 4, provided that the amino acid residue bound to the C-terminal side of the catalytic histidine is not glutamic acid;
- (b) combining the set of reaction components under suitable reaction conditions whereby peroxycarboxylic acid is produced;
- and
- (c) optionally diluting the peroxycarboxylic acid produced in step (b).
23 more elements
A composition comprising:
- (a) a set of reaction components comprising:
- (1) at least one substrate selected from the group consisting of:
- (i) one or more esters having the structure
- [X]mR5
- wherein
- X=an ester group of the formula R6—C(O)O;
- R6=a C1 to C7 linear, branched or cyclic hydrocarbyl moiety, optionally substituted with hydroxyl groups or C1 to C4 alkoxy groups, wherein R6 optionally comprises one or more ether linkages for R6=C2 to C7;
- R5=a C1 to C6 linear, branched, or cyclic hydrocarbyl moiety or a five-membered cyclic heteroaromatic moiety or six-membered cyclic aromatic or heteroaromatic moiety optionally substituted with hydroxyl groups;
- wherein each carbon atom in R5 individually comprises no more than one hydroxyl group or no more than one ester group or carboxylic acid group;
- wherein R5 optionally comprises one or more ether linkages;
- m=an integer ranging from 1 to the number of carbon atoms in R5;
- and
- wherein said esters have solubility in water of at least 5 ppm at 25° C.;
- (ii) one or more glycerides having the structure
- wherein R1=a C1 to C21 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R3 and R4 are individually H or R1C(O);
- (iii) one or more esters of the formula:
- wherein R1=a C1 to C7 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R2=a C1 to C10 straight chain or branched chain alkyl, alkenyl, alkynyl, aryl, alkylaryl, alkylheteroaryl, heteroaryl, (CH2CH2O)n, or (CH2CH(CH3)—O)nH and n is 1 to 10;
- (iv) one or more acylated monosaccharides, acylated disaccharides, or acylated polysaccharides;
- and
- (v) any combination of (i) through (iv);
- (2) a source of peroxygen;
- and
- (3) an enzyme catalyst comprising a polypeptide having perhydrolytic activity and an amino acid sequence having at least 90% identity to the amino acid sequence set forth in SEQ ID NO:
- 4, provided that the amino acid residue bound to the C-terminal side of the catalytic histidine is not glutamic acid;
- and
- (b) at least one peroxycarboxylic acid formed upon combining the set of reaction components of (a).
23 more elements
A peracid generation and delivery system comprising:
- (a) a first compartment comprising
- (1) an enzyme catalyst comprising a polypeptide having perhydrolytic activity and an amino acid sequence having at least 90% identity to the amino acid sequence set forth in SEQ ID NO:
- 4, provided that the amino acid residue bound to the C-terminal side of the catalytic histidine is not glutamic acid;
- (2) at least one substrate selected from the group consisting of:
- (i) one or more esters having the structure
- [X]mR5
- wherein
- X=an ester group of the formula R6—C(O)O;
- R6=a C1 to C7 linear, branched or cyclic hydrocarbyl moiety, optionally substituted with hydroxyl groups or C1 to C4 alkoxy groups, wherein R6 optionally comprises one or more ether linkages for R6=C2 to C7;
- R5=a C1 to C6 linear, branched, or cyclic hydrocarbyl moiety or a five-membered cyclic heteroaromatic moiety or six-membered cyclic aromatic or heteroaromatic moiety optionally substituted with hydroxyl groups;
- wherein each carbon atom in R5 individually comprises no more than one hydroxyl group or no more than one ester group or carboxylic acid group;
- wherein R5 optionally comprises one or more ether linkages;
- m=an integer ranging from 1 to the number of carbon atoms in R5;
- and
- wherein said esters have solubility in water of at least 5 ppm at 25° C.;
- (ii) one or more glycerides having the structure
- wherein R1=a C1 to C21 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R3 and R4 are individually H or R1C(O);
- (iii) one or more esters of the formula:
- wherein R1=a C1 to C7 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R2=a C1 to C10 straight chain or branched chain alkyl, alkenyl, alkynyl, aryl, alkylaryl, alkylheteroaryl, heteroaryl, (CH2CH2O)n, or (CH2CH(CH3)—O)nH and n is 1 to 10;
- (iv) one or more acylated monosaccharides, acylated disaccharides, or acylated polysaccharides;
- and
- (v) any combination of (i) through (iv);
- and
- (3) an optional buffer;
- and
- (b) a second compartment comprising
- (1) source of peroxygen;
- (2) a peroxide stabilizer;
- and
- (3) an optional buffer.
27 more elements
An isolated polypeptide having perhydrolytic activity comprising the amino acid sequence of SEQ ID NO:
- 14 or SEQ ID NO:
- 16.
A laundry care composition comprising
- a) a polypeptide having perhydrolytic activity and an amino acid sequence having at least 90% identity to the amino acid sequence set forth in SEQ ID NO:
- 4, provided that the amino acid residue bound to the C-terminal side of the catalytic histidine is not glutamic acid;
- b) at least one substrate selected from the group consisting of:
- (i) one or more esters having the structure
- [X]mR5
- wherein
- X=an ester group of the formula R6—C(O)O;
- R6=a C1 to C7 linear, branched or cyclic hydrocarbyl moiety, optionally substituted with hydroxyl groups or C1 to C4 alkoxy groups, wherein R6 optionally comprises one or more ether linkages for R6=C2 to C7;
- R5=a C1 to C6 linear, branched, or cyclic hydrocarbyl moiety or a five-membered cyclic heteroaromatic moiety or six-membered cyclic aromatic or heteroaromatic moiety optionally substituted with hydroxyl groups;
- wherein each carbon atom in R5 individually comprises no more than one hydroxyl group or no more than one ester group or carboxylic acid group;
- wherein R5 optionally comprises one or more ether linkages;
- m=an integer ranging from 1 to the number of carbon atoms in R5;
- and
- wherein said esters have solubility in water of at least 5 ppm at 25° C.;
- (ii) one or more glycerides having the structure
- wherein R1=a C1 to C21 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R3 and R4 are individually H or R1C(O);
- (iii) one or more esters of the formula:
- wherein R1=a C1 to C7 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R2=a C1 to 010 straight chain or branched chain alkyl, alkenyl, alkynyl, aryl, alkylaryl, alkylheteroaryl, heteroaryl, (CH2CH2O)n, or (CH2CH(CH3)—O)nH and n is 1 to 10;
- (iv) one or more acylated monosaccharides, acylated disaccharides, or acylated polysaccharides;
- and
- (v) any combination of (i) through (iv);
- and
- c) a source of peroxygen;
- and
- d) at least one surfactant.
22 more elements
A personal care product comprising a polypeptide having perhydrolytic activity, said polypeptide having an amino acid sequence having at least 90% identity to the amino acid sequence set forth in SEQ ID NO:
- 4, provided that the amino acid residue bound to the C-terminal side of the catalytic histidine is not glutamic acid.
Dependencies are inferred from the claim text.