Method for producing virus vector for gene transfer
AssigneesMie University, Biocomo Inc.
InventorsMasayuki Fukumura, Mitsuo Kawano, Tetsuya Nosaka, Junpei Ohtsuka
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Abstract
The present invention discloses a cell system, as a host cell to be infected with an F gene-deficient virus, which can constitutively and stably express the F protein, and a method for producing an F gene-deficient virus by utilizing the cell. A non-proliferative human parainfluenza type 2 virus vector is produced by co-culturing an F gene-deficient human parainfluenza type 2 virus with a Vero cell having the F gene of human parainfluenza type 2 virus in such a manner that the F gene is non-inducibly expressed, and isolating viral particles from a culture supernatant.
Claims3
A method for producing a non-proliferative human parainfluenza type 2 virus vector, comprising the steps of:
- co-culturing an F gene-deficient human parainfluenza type 2 virus with a Vero cell non-inducibly expressing the F gene of human parainfluenza type 2 virus, and isolating viral particles from a culture supernatant.
An isolated Vero cell that is non-inducibly expressing the F gene of human parainfluenza type 2 virus.
Dependencies are inferred from the claim text.