Minicircle vector production
AssigneesGerhard Jechlinger, Peter Mayrhofer
InventorsPeter Mayrhofer, Wolfgang Jechlinger, Werner Lubitz
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Abstract
A plasmid is provided comprising the following functional units: a prokaryotic origin of replication, a marker sequence, two specific recombinase recognition sequences and a multiple cloning site, whereby it comprises a gene coding for a sequence specific recombinase, whereby the units are arranged on the plasmid in such a way that the plasmid is divided into a miniplasmid and a minicircle upon expression of the sequence specific recombinase, said miniplasmid comprising the prokaryotic origin of replication, the marker sequence and the gene for the sequence specific recombinase and said minicircle comprising the multiple cloning site.
Claims9
A method for isolating a miniplasmid or a minicircle, said miniplasmid or said minicircle comprising a recognition sequence which is a lac operator site, wherein said method comprises
- a) binding said miniplasmid or minicircle to a LacI repressor protein, and
- b) separating the bound minicircle or miniplasmid from concomitant material, thereby isolating or purifying the bound miniplasmid or minicircle.
Dependencies are inferred from the claim text.