Conditioned medium obtained from stem cells and its use in therapy
AssigneeMedStar Health Research Institute, Inc.
InventorsStephen E. Epstein, Mary Susan Burnett, Amir Najafi
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Abstract
Described herein are conditioned medium and processed conditioned medium, each of which comprises secreted stem cell factors; compositions containing conditioned medium and/or processed conditioned medium and a delivery polymer. The conditioned medium, processed conditioned medium and compositions may be used to promote blood vessel growth and healing of injured tissues.
Claims10
A method of producing processed conditioned medium, comprising:
- (a) culturing stem cells in growth-factor-free medium comprising desferroxamine, wherein the stem cells have been previously cultured in growth medium, thereby generating conditioned medium that comprises factors secreted by the stem cells;
- (b) harvesting the conditioned medium;
- and
- (c) filtering the conditioned medium to produce processed conditioned medium.
A method of enhancing secretion of angiogenic factors by stem cells, comprising culturing the stem cells in growth factor-free medium comprising desferroxamine.
Dependencies are inferred from the claim text.