Methods of detection of coccidioides species in bodily fluid
AssigneesArizona Board of Regents, A Body Corporate of the State of Arizona Acting for and On Behalf of Arizona State University, Mayo Foundation for Medical Education and Research
InventorsDouglas Lake, Kwasi Antwi, Janis E. Blair
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Abstract
Coccidioidomycosis (Valley Fever) is currently diagnosed by relying on infected individuals (humans, dogs, etc.) to generate antibodies against the fungus. Since the immune response against Valley Fever can be delayed or absent in many cases, methods that detect a polypeptide or peptide component of the Coccidioides sp. fungus in a bodily fluid such as blood are provided.
Claims2
A method for diagnosing Coccidioidomycosis comprising testing a fluid sample from a subject at risk of Coccidioidomycosis for presence of a polypeptide comprising the amino acid sequence PGLDSKSLACTFSQV (SEQ ID NO:
- 4), wherein the method comprises
- a) contacting said sample from a subject at risk of Coccidioidomycosis with an antibody selective for a polypeptide comprising the amino acid sequence PGLDSKSLACTFSQV (SEQ ID NO:
- 4), under conditions to promote antibody binding to its selective antigen;
- b) removing unbound antibodies;
- and
- c) detecting antibody-antigen complexes.
Dependencies are inferred from the claim text.