Detection of Johnsongrass and its hybrid seed
AssigneePioneer Hi-Bred International, Inc.
InventorsBenjamin Kaufman, Mulu Ayele, Olga Ellern
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Abstract
Methods for detecting the presence or amount of Johnsongrass or Johnsongrass hybrid genetic material in a biological sample, preferably the detection of the presence or amount of Johnsongrass or Johnsongrass hybrid seed in a seed sample, are disclosed. The methods involve detection of certain nucleic acid sequences, namely Johnsongrass detection sequences, optionally through a primer-based amplification process, such as PCR. Also disclosed are isolated polynucleotides, replication compositions and kits for carrying out the detection methods.
Claims2
A method of determining quality of Sorghum bicolor seed production, the method comprising:
- a. providing a biological sample comprising Sorghum bicolor seeds, wherein the biological sample is suspected of also containing Johnsongrass or Johnsongrass hybrid genetic material;
- b. providing the primer pair of SEQ ID NOS.:
- 76 and 77 or the probe of SEQ ID NO:
- 136 that selectively binds to the Johnsongrass or Johnsongrass hybrid genetic material to discriminate against related genetic material;
- c. performing a hybridization or an amplification reaction wherein the primer pair of SEQ ID NOS.:
- 76 and 77 or probe of SEQ ID NO:
- 136 binds to Johnsongrass or Johnsongrass hybrid genetic material and does not substantially bind to genetic material from Sorghum bicolor;
- and
- d. determining the quality of Sorghum bicolor seed production based on the presence or the amount of Johnsongrass or Johnsongrass hybrid genetic material.
Dependencies are inferred from the claim text.