Recombinant classical swine fever virus (CSFV) comprising a modified E2 protein and methods for generating said recombinant CSFV
AssigneeIntervet International B.V.
InventorsJeroen Alexander Kortekaas, Rianka Petronella Maria Vloet
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Abstract
The invention relates to a recombinant classical swine fever virus (CSFV). A preferred recombinant CSFV comprises a deletion of at least one amino acid in a “TAVSPTTLR” domain of the E2 protein. The invention further relates to a vaccine comprising the recombinant CSFV, a method for generating a recombinant CSFV, and use of a recombinant CSFV.
Claims16
An infectious replication competent recombinant classical swine fever virus (CSFV), comprising a deletion in a “TAVSPTTLR” (SEQ ID NO:
- 1) domain of the E2 protein of classical swine fever virus, corresponding to amino acid positions 829 to 837 of a parental CSFV polyprotein;
- wherein said deletion is selected from the group consisting of P833, T834, and P833 and T834.
A recombinant classical swine fever virus (CSFV), comprising a deletion of at least one amino acid in a “TAVSPTTLR” (SEQ ID NO:
- 1) domain of the E2 protein corresponding to position 829 to 837 of a parental CSFV polyprotein;
- and further comprising a silent mutation that is a U to C alteration at position 1549 in the ERNS gene.
A recombinant classical swine fever virus (CSFV), comprising a deletion of at least one amino acid in a “TAVSPTTLR” (SEQ ID NO:
- 1) domain of the E2 protein, corresponding to position 829 to 837 of a parental CSFV polyprotein that comprises at least one further alteration of the parental genome;
- wherein the at least one further alteration results in a glycine (G) at position 831, and/or a phenylalanine (F) at position 789, and/or a threonine (T) at position 445;
- and/or forms a N-linked glycosylation site in the E2 protein.
A cDNA molecule comprising an infectious replication competent recombinant classical swine fever virus (CSFV) genome encoding an E2 protein of classical swine fever virus, and comprising a deletion of at least one amino acid in a “TAVSPTTLR” (SEQ ID NO:
- 1) domain;
- wherein said deletion is selected from the group consisting of P833, T834, and P833 and T834.
Dependencies are inferred from the claim text.