Haemophilus parasuis polypeptides and methods of use
AssigneeRegents of the University of Minnesota
InventorSimone Oliveira
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Abstract
The present invention provides isolated polypeptides having oligopeptide permease activity and an amino acid sequence that has at least 80% identity with a Haemophilus parasuis OppA polypeptide. Also provided by the present invention are isolated polynucleotides that encode the polypeptides described herein, and antibody that specifically binds a polypeptide described herein. The present invention further provides genetically modified microbes, such as attenuated Haemophilus parasuis strains and other microbes that express polypeptides described herein. Also included are methods for using the polypeptides, polynucleotides, antibody, and genetically modified microbes.
Claims9
A method for detecting the presence of an antibody against Haemophilus parasuis in a biological sample from an animal, comprising:
- mixing the biological sample from the animal with a polypeptide under conditions suitable for formation of an antigen:
- antibody complex, said biological sample containing an antibody against Haemophilus parasuis, wherein the polypeptide comprises an amino acid sequence having at least 80% identity with SEQ ID NO:2, wherein the antibody binds to an oligopeptide permease OppA of Haemophilus parasuis as set forth in SEQ ID NO:2, wherein the polypeptide has oligopeptide permease activity, wherein the animal is selected from the group consisting of porcine, avian, bovine, caprine, ovine, equine, murine, human, and a companion animal;
- and
- detecting the complex, wherein the presence of the complex indicates the animal has antibody that specifically binds to the polypeptide.
A method for detecting the presence of an antibody against Haemophilus parasuis in a biological sample from an animal, comprising:
- mixing the biological sample from the animal with a polypeptide under conditions suitable for formation of an antigen:
- antibody complex, said biological sample containing an antibody against Haemophilus parasuis, wherein the polypeptide comprises an amino acid sequence having at least 80% identity with SEQ ID NO:2, wherein the antibody detected is an antibody that binds to an oligopeptide permease OppA of Haemophilus parasuis as set forth in SEQ ID NO:2, and does not bind to an oligopeptide permease of Actinobacillus pleuropneumoniae as set forth in SEQ ID NO:4 wherein the animal is selected from the group consisting of porcine, avian, bovine, caprine, ovine, equine, murine, human, and a companion animal;
- and
- detecting the complex, wherein the presence of the complex indicates the animal has antibody that specifically binds to the polypeptide.
Dependencies are inferred from the claim text.