High-affinity fully functional soluble single-domain human CD4, antibodies, and related fusion proteins
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsDimiter S. Dimitrov, Weizao Chen
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Abstract
The invention provides engineered antibody domains (eAds), a polypeptide comprising a single domain CD4, as well as a fusion protein comprising the same. Nucleic acids encoding eAd and/or polypeptide or the fusion protein thereof, as well as compositions or cells comprising the eAd, polypeptide, fusion protein, or nucleic acid also are provided.
Claims17
An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:
- 11, provided that the polypeptide does not comprise the amino acid sequence of SEQ ID NO:
- 12.
An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:
- 13 or SEQ ID NO:
- 14.
An isolated polypeptide comprising the amino acid sequence of KKVVx1x2x3x4GDTVELx5CTASQKKx6IQFx7WKx8SNQIKILGNQGSFLTKGPSKLNDRx9DSRRSLWDQGx10FPLIIKNLKx11EDSx12TYICEVEDQKEEVQLx13Vx14G (SEQ ID NO:
- 11), wherein
- x1 selected from the group consisting of I, Y, V, E, W, F, and T;
- x2 is G or A;
- x3 is K or Q;
- x4 is K or E;
- x5 is T or A;
- x6 is S or N;
- x7 is H or Q;
- x8 is N or D;
- x9 is A or V;
- x10 is N or S;
- x11 is selected from the group consisting of I, P, L, Y, V, S, and E;
- x12 is D or G;
- x13 is selected from the group consisting of L, I, V, H, and C;
- and
- x14 is selected from the group consisting of F, L, V, Q, R, I, and T.
Dependencies are inferred from the claim text.