Method for antibiotic susceptibility testing and determining minimum inhibitory concentration of the antibiotic
AssigneeNational Cheng Kung University
InventorsCheng-Che Chung, I-Fang Cheng, Wen-Horng Yang, Hsien-Chang Chang
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Abstract
A method of antibiotic susceptibility testing is disclosed, and includes the following steps: (A) providing a sample to be tested wherein the sample contains a microbe; (B) adding an antibiotic into the sample, wherein the antibiotic serves to inhibit cell wall synthesis; (C) checking the sample by dielectrophoresis and observing a shape change of the microbe; and (D) determining whether the microbe is susceptible to the antibiotic according to the shape change thereof. The present invention also discloses a method for determining a minimum inhibitory concentration of the antibiotic.
Claims8
A method of antibiotic susceptibility testing, comprising the following steps:
- (A) providing a test sample containing a microbe, wherein the microbe is a Gram-negative rod-shaped bacterium;
- (B) adding an antibiotic to the test sample, wherein the antibiotic is used to inhibit cell wall synthesis, and wherein the antibiotic is a β-lactam antibiotic;
- (C) executing dielectrophoresis to the test sample and observing morphologic changes of the microbe in the test sample;
- and
- (D) determining whether the microbe is resistant to the antibiotic according to the morphologic changes of the microbe.
A method for determining a minimum inhibitory concentration of an antibiotic, comprising the following steps:
- (A) providing a test sample containing a microbe, wherein the microbe is a Gram-negative rod-shaped bacterium;
- (B) adding an antibiotic with different concentrations to the test sample, wherein the antibiotic is used to inhibit cell wall synthesis, and wherein the antibiotic is a β-lactam antibiotic;
- (C) executing dielectrophoresis to the test sample and observing morphologic changes and crossover frequency changes of the microbe in the test sample;
- and
- (D) determining the minimum inhibitory concentration of the antibiotic according to the morphologic changes and the crossover frequency changes of the microbe.
Dependencies are inferred from the claim text.