Mycobacterial peptide deformylase
AssigneeCouncil of Scientific and Industrial Research
InventorsRahul Saxena, Pardip K. Chakraborti
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Abstract
The present invention relates to the design of the Antisense-oligonucleotide complementary to the specific region of peptide deformylase gene from Mycobacterium tuberculosis. The use of this Antisense-oligonucleotide on mycobacterial culture inhibits the production of the peptide deformylase enzyme by hybridizing within the region, which is found to be responsible for maintaining stability as well as retaining the functionality of the enzyme and thus in turn affecting the growth of the cells. This invention also establishes the essentiality of the peptide deformylase enzyme in mycobacteria and claims it as a drug target in this microorganism.
Claims18
An antisense oligonucleotide fully complementary to a polynucleotide sequence encoding a mycobacterial deformylase peptide insertion sequence XTXRRRGVVINP (SEQ ID NO:
- 13), wherein X can be any naturally occurring amino acid, or a peptide insertion sequence 90% to 95% similar to the corresponding peptide insertion sequence from M. tuberculosis, M. smegmatis, M. bovis, M. avium, or M. leprae, and wherein the antisense oligonucleotide is modified with phosphorothioates.
A method for inhibiting the activity and growth of mycobacteria the method comprising a step of administering an antisense oligonucleotide fully complementary to a polynucleotide sequence encoding a mycobacterial deformylase peptide insertion sequence XTXRRRGVVINP (SEQ ID NO:
- 13), wherein X can be any naturally occurring amino acid, or a peptide insertion sequence 90% to 95% similar to the corresponding peptide insertion sequence from M. tuberculosis, M. smegmatis, M. bovis, M. avium, or M. leprae.
A composition comprising an isolated and phosphorothioated antisense oligonucleotide fully complementary to a polynucleotide sequence encoding a mycobacterial deformylase peptide insertion sequence XTXRRRGVVINP (SEQ ID NO:
- 13), wherein X can be any naturally occurring amino acid, or a peptide insertion sequence 90% to 95% similar to the corresponding peptide insertion sequence from M. tuberculosis, M. smegmatis, M. bovis, M. avium, or M. leprae.
Dependencies are inferred from the claim text.