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US 8,906,619 B2Granted
HDV nucleic acid molecules, fragments and applications thereof
AssigneeAssistance Publique-Hopitaux de Paris
InventorsPaul Deny, Nadjia Radjef, Patricia Huc-Anais
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Abstract
The invention concerns nucleic acid molecules derived from novel hepatitis D virus strains or isolates constituting genotypes different from known I, II and III genotypes, their fragments, corresponding proteins and their uses as diagnostic reagents. The invention also concerns a method for sensitive diagnosis of the hepatitis D virus (or delta hepatitis virus) and a method for epidemiologic monitoring of HDV-related infections.
Claims5
A method for detection of a variant HDV, by hybridization and/or amplification, carried out from a biological sample,
- wherein the genome of said variant HDV encodes the LHD protein of SEQ ID NO:
- 27 or the sHD protein of SEQ ID NO:
- 29,
- which method comprises:
- (1) extracting the nucleic acid to be detected, belonging to the genome of the virus possibly present in the biological sample,
- (2) carrying out at least one gene amplification using a pair of primers selected from the group consisting of the primers capable of amplifying the region R2 of the HDV genomic RNA, and
- (3) analyzing the amplified product by comparison with the sequence of SEQ ID NO:
- 26, corresponding to the complete genome of the variant referred to as dFr644.
5 more elements
Dependencies are inferred from the claim text.