Biosensors for biological or chemical analysis and systems and methods for same

A biosensor comprising:

• a flow cell;
• a detection device comprising a plurality of stacked layers and having a detector surface configured to support reaction sites, the stacked layers including a filter layer and a solid-state imager coupled to the filter layer, the filter layer comprising filter walls and a light-absorbing material that is deposited between adjacent filter walls, the light-absorbing material configured to prevent transmission of excitation light and permit transmission of emission signals, wherein the adjacent filter walls define a detection path therebetween through the corresponding light-absorbing material toward the solid-state imager, the filter walls configured to reflect the emission signals;
• wherein the flow cell is mounted to the detector surface and defines a flow channel between at least one surface of the flow cell and the detection device, the at least one surface of the flow cell comprising a material that permits transmission of the excitation light.