Disease resistant cucumber plants

A method for selecting a plant having resistance to CYSDV and to cucumber powdery mildew, wherein said plant is of the species Cucumis sativus, said method comprising performing a marker detection assay, wherein said assay comprises the steps of:

• a) obtaining a sample of nucleic acid from said plant;
• b) detecting in the sample from said plant the presence of at least one marker linked to CYSDV resistance selected from the group consisting of markers E16/M50-244, E16/M50-188, and E11/M48-251,
• c) detecting in the sample from said plant the presence of at least one marker linked to powdery mildew resistance selected from the group consisting of:
• the single nucleotide polymorphism marker 39T→G in SEQ ID NO:1,
• the single nucleotide polymorphism marker 29G→A in SEQ ID NO:2, and
• markers E16/M50-F-194, E11/M48-F-251, and E23/M38-M001,
• or is selected from the group consisting of:
• the single nucleotide polymorphism marker 193C→T in SEQ ID NO:3,
• the insertion mutation 5′-AATTT-3′ at position 221 in SEQ ID NO:4,
• markers E23/M40-M003, E24/M46-M002, E24/M46-M003, E12/M91-M003, E26/M43-M003, E14/M59-F-134 and E14/M59-F-200.

A method of producing a plant of the species Cucumis sativus which plant exhibits resistance to CYSDV and to cucumber powdery mildew, comprising the steps of:

• a) selecting a first cucumber plant that comprises a QTL that confers resistance CYSDV, by detecting in the genome of said plant the presence of at least one marker linked to the CYSDV resistance-conferring QTL indicated by markers E16/M50-244, E16/M50-188, and E11/M48-251;
• b) selecting a second cucumber plant that comprises at least one QTL that confers resistance to cucumber powdery mildew
• by detecting in the genome of said plant the presence of at least one marker linked to a first cucumber powdery mildew resistance-conferring QTL indicated by the following markers:
• the single nucleotide polymorphism marker 39T→G in SEQ ID NO:1,
• the single nucleotide polymorphism marker 29G→A in SEQ ID NO:2, and
• markers E16/M50-F-194, E11/M48-F-251, and E23/M38-M001;
• or
• by detecting in the genome of said plant the presence of at least one marker linked to a second cucumber powdery mildew resistance-conferring QTL indicated by the following markers:
• the single nucleotide polymorphism marker 193C→T in SEQ ID NO:3,
• the insertion mutation 5′-AATTT-3′ at position 221 in SEQ ID NO:4, and
• markers E23/M40-M003, E24/M46-M002, E24/M46-M003, E12/M91-M003, E26/M43-M003, E14/M59-F-134 and E14/M59-F-200;
• c) crossing said plants from step a) and step b) to produce F1 seeds;
• d) growing an amount of F1 seeds into F1 plants, generating a further offspring population from said F1 plants by crossing or selfing, and
• e) selecting from among said further offspring plants a plant that comprises at least one marker linked to the CYSDV resistance-conferring QTL as defined in step a) and at least one marker linked to the cucumber powdery mildew resistance-conferring QTL as defined in step b).