Viral promoter, truncations thereof, and methods of use
AssigneesPioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and Company
InventorsScott Diehn, Albert L. Lu, Carl R. Simmons
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Abstract
The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
Claims29
A DNA construct comprising a promoter consisting of the nucleotide sequence of SEQ ID NO:
- 1 and a heterologous nucleotide sequence of interest operably linked to the promoter.
A DNA construct comprising a promoter consisting of the nucleotide sequence of SEQ ID NO:
- 2 and a heterologous nucleotide sequence of interest operably linked to the promoter.
An isolated nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:
- 3.
A method for expressing a nucleotide sequence in a plant, said method comprising introducing into a plant a DNA construct, said DNA construct comprising a promoter nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence consisting of the sequence set forth in SEQ ID NO:
- 2;
- b) a nucleotide sequence consisting of the sequence set forth in SEQ ID NO:
- 1;
- and
- c) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 3;
- and
- a heterologous nucleotide sequence of interest operably linked to said promoter.
A method for expressing a nucleotide sequence in a plant cell, said method comprising introducing into a plant cell a DNA construct comprising a promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence consisting of the sequence set forth in SEQ ID NO:
- 2;
- b) a nucleotide sequence consisting of the sequence set forth in SEQ ID NO:
- 1;
- and
- c) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 3;
- and
- a heterologous nucleotide sequence of interest operably linked to said promoter.
A method for selectively expressing a nucleotide sequence in maize green plant tissues, said method comprising introducing into a plant cell a DNA construct comprising a promoter nucleotide sequence selected from the group consisting of:
- a) the nucleotide sequence consisting of the sequence set forth in SEQ ID NO:
- 2,
- b) the nucleotide sequence consisting of the sequence set forth in SEQ ID NO:
- 1, and
- c) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 3;
- and
- a heterologous nucleotide sequence of interest operably linked to said promoter;
- and
- regenerating a transformed plant from said plant cell.
Dependencies are inferred from the claim text.