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US 8,895,706 B2Granted
Chromatography matrices including novel Staphylococcus aureus protein A based ligands
AssigneeEMD Millipore Corporation
InventorsShari Spector, Robert Smith, Joe Orlando, Nanying Bian
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Abstract
The present invention relates to chromatography matrices including ligands based on one or more domains of immunoglobulin-binding proteins such as, Staphylooccus aureus Protein A (SpA), as well as methods of using the same.
Claims11
An affinity chromatography matrix comprising:
- (a) a solid support;
- and
- (b) an affinity chromatography ligand attached to the solid support, wherein the ligand comprises one or more domains based on the amino acid sequence set forth in SEQ ID NO:
- 4 or the amino acid sequence encoded by the nucleotide sequence set forth in SEQ ID NO. 10, and having a deletion of at least 3 consecutive amino acids from the N-terminus starting at position 1 or position 2 of the amino acid sequence set forth in SEQ ID NO:4 or the amino acid sequence encoded by SEQ ID NO:10.
1 more element
An affinity chromatography ligand comprising the structure:
- [(X)n,(Y)m]n+m,
- where X represents a C domain of SpA,
- n represents the number of domains randing from zero through (m−1),
- Y represents a C domain of SpA having at least 3 consecutive amino acids deleted from the N-terminus, and
- m represents the number of Y domains ranging from one through eight, wherein the ligand is attached to a solid support via multipoint attachment.
2 more elements
Dependencies are inferred from the claim text.