Modified human tumor necrosis factor receptor-I polypeptide or fragment thereof, and method for preparing same
AssigneeHanall Biopharma Co., Ltd.
InventorsSung Wuk Kim, Sung Soo Jun, Seung Kook Park, Song Young Kim, Eun Sun Kim, Jae Kap Jeong, Ha Na Kim, Yeon Jung Song
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Abstract
The present invention relates to a modified human tumor necrosis factor receptor-1 polypeptide which is capable of binding to a tumor necrosis factor in vivo or ex vivo, or to a fragment thereof. The modified human tumor necrosis factor receptor-1 polypeptide or the fragment thereof according to the present invention exhibit improved binding affinity to the tumor necrosis factor.
Claims18
A modified human tumor necrosis factor receptor-I (TNFRI) polypeptide or fragment thereof comprising an amino acid sequence selected from the group consisting of:
- i) the amino acid sequence of natural TNFRI as set forth in SEQ ID NO:
- 1;
- ii) the amino acid sequence composed of amino acid residues 41 to 211 of the amino acid sequence of the natural TNFRI set forth in SEQ ID NO:
- 1 (TNFRI171);
- iii) the amino acid sequence composed of amino acid residues 41 to 166 of the amino acid sequence of the natural TNFRI set forth in SEQ ID NO:
- 1 (TNFRI126);
- and
- iv) the amino acid sequence composed of amino acid residues 41 to 145 of the amino acid sequence of the natural TNFRI set forth in SEQ ID NO:
- 1 (TNFRI105);
- wherein the amino acid sequence is modified at least at positions 92, 95, 97 and 98.
A modified TNFRI polypeptide having sequence homology of more than 90%, more than 95%, more than 96%, more than 97%, more than 98% or more than 99% to the amino acid sequence as set forth in SEQ ID NO:
- 1, the modified TNFRI polypeptide or a fragment thereof comprising modifications corresponding to positions 92, 95, 97 and 98 in the amino acid sequence,
- wherein the amino acid modification at each position is:
- i) substitution of S with I, F, M, W, Y, K or H position 92;
- ii) substitution of H with F at position 95;
- iii) substitution of R with P, L or I at position 97;
- and
- iv) substitution of H with A or G at position 98.
Dependencies are inferred from the claim text.