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US 8,895,321 B2Granted
Integrated affinity microcolumns and affinity capillary electrophoresis
AssigneeStc.Unm
InventorsGabriel Lopez, Linnea Ista, Steven RJ Brueck, Aurelio Evangelista Lara, Mangesh Bore
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Abstract
Device and method for detecting the presence of known or unknown toxic agents in a fluid sample. Targets in the sample are bound to releasable receptors immobilized in a reaction region of a micro- or nano-fluidic device. The receptors are selected based on their affinity for classes of known toxic agents. The receptors are freed and the bound and unbound receptors separated based on differential electrokinetic mobilities while they travel to a detection device.
Claims5
A method for detecting the presence of a target in a fluid sample, the method comprising:
- introducing a fluid sample to a microfluidic device including a reaction region, a detection region, and a microfluidic channel therebetween;
- exposing the fluid sample to a plurality of labeled receptors reversibly immobilized within the reaction region under conditions suitable for the receptors to specifically bind any target present in the fluid sample;
- releasing the receptors from the reaction region under conditions such that a substantial portion of the bound target remains bound to the released receptors;
- transporting the released receptors through the microfluidic channel such that bound and unbound receptors separate based on differential electrokinetic mobilities;
- and
- detecting a signal produced by the arrival of the bound and unbound receptors to the detection region.
3 more elements
Dependencies are inferred from the claim text.