Reagent for measuring active nitrogen
AssigneeThe University of Tokyo
InventorsTetsuo Nagano, Yasuteru Urano, Saki Izumi
Life of this patent
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Abstract
A compound represented by the general formula (I) [R1 and R2 are amino groups that substitute at adjacent positions on the benzene ring; R3 and R4 are halogen atoms; R5 and R6 represent hydrogen atom, an acyl group or an acyloxy(C1-6 alkyl) group; R7 and R8 represent —(CH2)p—N(R9)(R10) (p is 1 to 4, and R9 and R10 represent —(CH2)n—COOH (n is 1 to 4))], which is useful for measuring a reactive nitrogen species existing in cells such as nitrogen monoxide or peroxynitrite at high sensitivity over a long period of time.
Claims6
A compound or a salt thereof represented by the following formula (A):
A compound represented by the following formula (B):
- wherein AM is an acetoxymethyl group.
A method for measuring a reactive nitrogen species, which comprises:
- (1) reacting a compound or a salt thereof represented by the following formula (A):
- with a reactive nitrogen species to form a fluorescently detectable triazole compound;
- and
- (2) detecting the fluorescently detectable triazole compound.
Dependencies are inferred from the claim text.