Nanofluidic platform for single mitochondria analysis
AssigneeThe Regents of the University of California
InventorsPeter Burke, Katayoun Zand, Ted Pham, Antonio Davila, Douglas Wallace
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Abstract
A microfluidic device for mitochondria analysis includes an inlet coupled to a first access channel, an outlet coupled to a second access channel, and a plurality of trapping channels fluidically coupled at one end to the first access channel and fluidically coupled at an opposing end to the second access channel, each trapping channel has a cross-sectional dimension about 2 μm in one direction and a cross-sectional dimension between about 0.45 and about 0.75 μm in a second direction.
Claims17
A microfluidic device for mitochondria analysis comprising:
- an inlet coupled to a first access channel;
- an outlet coupled to a second access channel;
- and
- a plurality of trapping channels fluidically coupled at one end to the first access channel and fluidically coupled at an opposing end to the second access channel, each trapping channel comprises a cross-sectional dimension about 2 μm in one of width or height and a cross-sectional dimension between about 0.45 and about 0.75 μm in the other of width or height.
A method of analyzing mitochondria comprising:
- flowing a sample containing fluorescently labeled mitochondria through a plurality of trapping channels, with each trapping channel having a cross-sectional dimension about 2 μm in one of width or height and a cross-sectional dimension between about 0.45 and about 0.75 μm in the other of width or height;
- trapping one or more mitochondria within the plurality of trapping channels;
- and
- imaging the trapped one or more mitochondria over a period of time.
Dependencies are inferred from the claim text.