US 8,895,300 B2Granted

Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof

AssigneeViaCyte, Inc.

InventorThomas C Schulz

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Abstract

The present invention relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation thereof.

Claims22

Dependent claims

A roller bottle comprising primate pluripotent stem cell (pPSC) aggregates in suspension in a physiologically acceptable medium, wherein the pPSC aggregates are formed in the roller bottle.

Dependencies are inferred from the claim text.

Facts

Application: 13/672,688Filed 8 Nov 2012 · continuation in part
Priority: 4 Nov 2008
Published: US 2013/0115695 A19 May 2013
Granted: 25 Nov 2014
Term ends: Not estimatedThe source records don’t support an estimate.

Classification19 codes

C12N 5/0606
Embryonic cells ; Embryoid bodies: pluripotent embryonic cells, e.g. embryonic stem cells [ES]
C12M 27/12
Rotating vessel: roller bottles; Roller tubes
C12N 5/0603
Vertebrate cells: embryonic cells ; Embryoid bodies
C12N 5/0676
Vertebrate cells: pancreatic cells

Family

Application12/264,760Parent of this continuation-in-part · filed 4 Nov 2008
Application13/220,590Parent of this continuation-in-part · filed 29 Aug 2011
US 2013/0115695 A1Pre-grant publication · published 9 May 2013
US 8,445,273 B2Parent of this continuation-in-part
US 8,008,075 B2Parent of this continuation-in-part
US 2010/0112691 A1Published application
US 2012/0045830 A1Published application
US 8,895,300 B2This patent

From the US filing history. Foreign family members aren’t listed.

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