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US 8,895,299 B2Granted
Method for expansion of stem cells
AssigneeXON Cells, Inc.
InventorNeil H. Riordan
Life of this patent
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Abstract
A method of increasing the growth of stem cells by mixing the stem cells with a growth medium that has been conditioned by an incubation with placental tissue. The method increases the expansion of the stem cell population.
Claims6
A method for the expansion or growth of stem cells without substantially inducing differentiation, the method comprising:
- collecting cord blood from a human umbilical cord;
- isolating CD34+ cells from the cord blood;
- producing live placenta conditioned medium (LPCM) by incubating whole human placenta in a Dulbecco's modified Eagle medium (DMEM) in a sterile container, wherein the whole placenta is submerged;
- isolating the LPCM;
- and
- contacting at least one CD34+ cell with a supplemented DMEM comprising the LPCM and a cytokine cocktail comprising 20 ng/ml IL-3, 250 ng/ml IL-6, 10 ng/ml stem cell factor, 250 ng/ml thrombopoietin, and 100 ng/ml Flt-3L, wherein the concentration of the LPCM in the supplemented DMEM is 50%.
3 more elements
Dependencies are inferred from the claim text.