Methods and systems of growing and harvesting cells in a hollow fiber bioreactor system with control conditions

A method of controlling a process in a protocol for use with a closed cell expansion system, the method comprising:

• providing the closed cell expansion system;
• providing a fluid conveyance assembly, wherein the fluid conveyance assembly comprises a bioreactor;
• engaging the fluid conveyance assembly;
• providing an air removal chamber, wherein the air removal chamber is mounted on the fluid conveyance assembly, and wherein the air removal chamber comprises a fluid containment chamber, the fluid containment chamber comprising:
• a fluid entrance aperture;
• a fluid exit aperture, wherein the fluid exit aperture is coupled to a fluid exit tube;
• and
• a vent aperture, wherein the vent aperture is located above the fluid entrance aperture and the fluid exit aperture;
• providing a pump to pump a fluid through the fluid entrance aperture and into the fluid containment chamber of the air removal chamber;
• initiating a first process of a first protocol, the first process comprising a load reagent step from the first protocol, wherein the first protocol comprises coating the bioreactor, and wherein the initiating comprises:
• operating the pump to pump the fluid through the fluid entrance aperture and into the fluid containment chamber, wherein the fluid reaches a fluid level in the fluid containment chamber;
• allowing the fluid to pass through the fluid exit aperture to enter the fluid exit tube;
• providing a first sensor to detect the fluid level in the fluid containment chamber;
• detecting the fluid level using the first sensor;
• providing a stop condition for the first process of the first protocol, wherein the stop condition for the first process of the first protocol involves the air removal chamber, comprising:
• meeting the stop condition for the first process of the first protocol when the fluid level in the fluid containment chamber reaches a predetermined level as detected by the first sensor;
• and
• when the fluid level in the fluid containment chamber is at the predetermined level, stopping the first process of the first protocol based on meeting the stop condition;
• initiating a second process of the first protocol, wherein the second process comprises an air removal chamber chase step from the first protocol, and wherein a stop condition for the second process of the first protocol involves the air removal chamber;
• and
• initiating a third process of the first protocol, wherein the third process comprises a circulate step from the first protocol, and wherein a stop condition for the third process of the first protocol involves a selected time or a manual stop.