Methods of producing isoprene and a co-product
AssigneesDanisco US Inc., The Goodyear Tire & Rubber Company
InventorsGopal K. Chotani, Caroline M. Peres, Derek H. Wells, Karl J. Sanford, Joseph C. McAuliffe
Life of this patent
Estimating the term…
Abstract
The invention features methods producing isoprene and a co-product, such as ethanol, 1,3-propanediol, or hydrogen from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides compositions comprising isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen. Additionally, the invention provides methods of co-producing isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen by culturing cells under conditions suitable for co-production of isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen.
Claims10
Recombinant host cells comprising (a) a heterologous nucleic acid encoding an isoprene synthase polypeptide, (b) a nucleic acid encoding an isopentenyl-diphosphate delta-isomerase (IDI) polypeptide, (c) nucleic acid(s) encoding a 1-Deoxyxylulose-5-phosphate synthase (DXS) polypeptide and/or one or more mevalonate (MVA) pathway polypeptides and (d) nucleic acid(s) encoding one or more propanediol pathway polypeptides,
- wherein said cells co-produce isoprene and 1,3-propanediol under oxygen-limited culture conditions, wherein the oxygen transfer rate (OTR) is less than the oxygen uptake rate (OUR).
Dependencies are inferred from the claim text.