Modified ethylenediamine-N, N′-disuccinate: ethylenediamine lyase
AssigneeMitsubishi Rayon Co., Ltd.
InventorsTakanori Akiyama, Wataru Mizunashi, Tetsuji Nakamura
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Abstract
The present invention provides a modified ethylenediamine-N,N′-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity.
Claims13
A recombinant vector comprising an isolated gene encoding a protein comprising the amino acid sequence of SEQ ID NO:1 and having an ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity.
An isolated gene encoding a modified protein comprising the amino acid sequence of SEQ ID NO:1 having 1 to 10 substitutions, and wherein the protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
An isolated gene encoding a modified protein comprising the amino acid sequence of SEQ ID NO:1 having 2 to 10 substitutions, wherein the substitutions include the substitution of the lysine residue at position 120 with a glutamic acid residue, and wherein the protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
An isolated gene encoding a modified protein comprising the amino acid sequence of SEQ ID NO:1 having 2 to 10 substitutions, wherein the substitutions include the substitution of the isoleucine residue at position 166 with a serine residue, and the substitution of the alanine residue at position 365 with a valine residue, and wherein the protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
An isolated gene encoding a modified protein comprising the amino acid sequence of SEQ ID NO:1 having 2 to 10 substitutions, wherein the substitutions include the substitution of the isoleucine residue at position 166 with a threonine residue, and the substitution of the alanine residue at position 365 with a valine residue, and wherein the protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
An isolated gene encoding a modified protein comprising the amino acid sequence of SEQ ID NO:1 having 3 to 10 substitutions, wherein the substitutions include the substitution of the lysine residue at position 120 with a glutamic acid residue, and the substitution of the isoleucine residue at position 166 with a serine residue and the substitution of the alanine residue at position 365 with a valine residue, and wherein the protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
An isolated gene encoding a modified protein comprising the amino acid sequence of SEQ ID NO:1 having 3 to 10 substitutions, wherein the substitutions include the substitution of the lysine residue at position 120 with a glutamic acid residue, and the substitution of the isoleucine residue at position 166 with a threonine residue and the substitution of the alanine residue at position 365 with a valine residue, and wherein the protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
Dependencies are inferred from the claim text.