Nucleic acid modifying enzymes
AssigneeBio-Rad Laboratories, Inc.
InventorYan Wang
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Abstract
This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.
Claims20
A polypeptide comprising a nuclease domain joined to a sequence non-specific double-stranded nucleic acid binding domain, wherein the sequence non-specific double-stranded nucleic acid binding domain has at least 75% identity to SEQ ID NO:2 or to amino acids 7-71 of SEQ ID NO:10.
A recombinant fusion polypeptide comprising at least two heterologous domains wherein a first domain that is a sequence-non-specific double-stranded nucleic-acid-binding domain that comprises an amino sequence that has at least 75% identity to SEQ ID NO:2 or to amino acids 7-71 of SEQ ID NO:10, is joined to a second domain that is a catalytic nucleic-acid-modifying domain, wherein the catalytic nucleic acid-modifying domain is a polymerase and where the presence of the sequence non-specific double-stranded nucleic acid binding domain stabilizes the double-stranded conformation of the nucleic acid.
Dependencies are inferred from the claim text.