Production of volatile dienes by enzymatic dehydration of light alkenols
AssigneeScientist of Fortune, S.A.
InventorPhillippe Marliere
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Abstract
Described is a method for generating conjugated dienes through a biological process. More specifically, the application describes a method for producing conjugated dienes (for example butadiene, isoprene or dimethylbutadiene) from light alkenols via enzymatic dehydration, in particular by making use of an alkenol dehydratase.
Claims29
A method for producing 1,3-butadiene comprising enzymatically converting a C4H8O alcohol to 1,3-butadiene, wherein said C4H8O alcohol is but-2-en-1-ol, but-3-en-2-ol, or but-3-en-1-ol, by an enzyme at least 70% identical to the amino acid sequence of SEQ ID NO:1, wherein said enzyme has alkenol dehydratase activity.
A composition comprising a microorganism which comprises an enzyme at least 70% identical to the amino acid sequence of SEQ ID NO:1, wherein said enzyme has alkenol dehydratase activity, and a C4H8O alcohol, wherein said C4H8O alcohol is but-2-en-1-ol, but-3-en-2-ol, or but-3-en-1-ol.
A composition comprising an enzyme at least 70% identical to the amino acid sequence of SEQ ID NO:1, wherein said enzyme has alkenol dehydratase activity, and a C4H8O alcohol, wherein said C4H8O alcohol is but-2-en-1-ol, but-3-en-2-ol, or but-3-en-1-ol.
Dependencies are inferred from the claim text.