Microorganism variants having hydrocarbon producing ability and method for producing hydrocarbon using the same

A mutant E. coli having the ability to produce alkanes selected from the group consisting of pentadecane, heptadecane, octane, and nonane, wherein said mutant E. coli is constructed by deletion or attenuation of a gene encoding acyl coenzyme A dehydrogenase and a fadR gene encoding a DNA-binding transcriptional dual regulator, and the native promoter of the gene encoding acyl-CoA synthetase and the attenuator-containing native promoter of the gene encoding the fatty acid outer membrane transporter are further substituted with a strong promoter selected from the group consisting of trc promoter, tac promoter, T7 promoter, lac promoter, and trp promoter in the mutant E. coli;

• and wherein said mutant E. coli is constructed by introduction or amplification of a gene encoding acyl-CoA thioesterase, an atoDA gene encoding acetyl-CoA:
• acetoacetyl-CoA transferase, a gene encoding fatty acyl-CoA reductase, a gene encoding fatty aldehyde decarbonylase, and a atoE gene encoding a short-chain fatty acid outer membrane transporter.

A mutant E. coli having the ability to produce alkanes selected from the group consisting of pentadecane, heptadecane, octane, and nonane, wherein said mutant E. coli is constructed by deletions or attenuation of a gene encoding acyl coenzyme A dehydrogenase and a fadR gene encoding a DNA-binding transcriptional dual regulator, and wherein the native promoter of the fabDHG operon and the native promoter of fabA gene are further substituted with a strong promoter selected from the group consisting of trc promoter, tac promoter, T7 promoter, lac promoter, and trp promoter in the mutant E. coli, the mutant E. coli having introduced or amplified therein a gene encoding acyl-CoA thioesterase, an atoDA gene encoding acetyl-CoA:

• acetoacetyl-CoA transferase, a gene encoding fatty acyl-CoA reductase, a gene encoding fatty aldehyde decarbonylase, and a atoE gene encoding a short-chain fatty acid outer membrane transporter.

A method for preparing a microorganism variant having the ability to produce alkanes selected from the group consisting of pentadecane, heptadecane, octane, and nonane, wherein the microorganism variant is constructed by deletions of a gene encoding acyl coenzyme A dehydrogenase and a fadR gene;

• and by introduction or amplification of a gene encoding an acyl-CoA thioesterase, an atoDA gene encoding an acetyl-CoA:
• acetoacetyl-CoA transferase, a gene encoding a fatty acyl-CoA reductase, a gene encoding a fatty aldehyde decarbonylase, and a atoE gene encoding a short-chain fatty acid outer membrane transporter;
• and by substitution of the native promoter of the gene encoding acyl-CoA synthetase and the attenuator-containing native promoter of the gene encoding the fatty acid outer membrane transporter with a strong promoter selected from the group consisting of trc promoter, tac promoter, T7 promoter, lac promoter, and trp promoter.